... | @@ -165,6 +165,6 @@ To setup a continuous synthesis simulation, we use the corresponding mRNA sequen |
... | @@ -165,6 +165,6 @@ To setup a continuous synthesis simulation, we use the corresponding mRNA sequen |
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Once the nascent chain is fully synthesized, the bond between NC:*l*@CA and tRNA:76@R is removed, as well as the corresponding bonding interaction energy terms. The nascent chain is ejected from the exit tunnel and then dissociated from the ribosome surface. We identify the complete dissociation as the minimum distance between the nascent chain and the ribosome surface keeps longer than 20 Å for at least 750 ps. The final nascent chain structure can be used to study the off-ribosome post-translational folding.
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Once the nascent chain is fully synthesized, the bond between NC:*l*@CA and tRNA:76@R is removed, as well as the corresponding bonding interaction energy terms. The nascent chain is ejected from the exit tunnel and then dissociated from the ribosome surface. We identify the complete dissociation as the minimum distance between the nascent chain and the ribosome surface keeps longer than 20 Å for at least 750 ps. The final nascent chain structure can be used to study the off-ribosome post-translational folding.
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Below is a video of the entire continuous synthesis process using this protocol for synthesizing Firfly luciferase on the *E.coli* ribosome (time is shown in the experimental timescale):
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Below is a video of the entire continuous synthesis process using this protocol for synthesizing Firfly luciferase (550 residue long) on the *E.coli* ribosome (time is shown in the experimental timescale):
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